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Current Problems in Cancer
journal homepage: www.elsevier.com/locate/cpcancer
Aberrant hypermethylation of OGDHL gene promoter in sporadic colorectal cancer
Mohammad Khalaj-kondori a,∗, Mina Hosseinnejad b, Asghar Hosseinzadeh b, Shahin Behroz Sharif c,d, Shahriar Hashemzadeh e a Dept. of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran b Department of Biology, Tabriz Branch of Islamic Azad University, Tabriz, Iran c Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran d Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran e Department of General & Vascular Surgery, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Background: Aberrant methylation patterns of certain genes including tumor suppressors, a major epige-netic event, contribute mainly to tumorigenesis. Promoter CpG island methylation in Oxoglutarate dehydro-genase like (OGDHL) gene has been reported to reduce gene AZD-5991 and hence apoptosis induction. This gene has been shown to be involved in colorectal cancer progression. In the present study, we investigated methylation status of OGDHL gene promoter in patients with colorectal cancer and evaluated its potential as a diagnostic biomarker.
Methods and material: After collecting clinicopathologic data of patients, tumor and matched tumor free margin samples were obtained from 40 individuals; total genomic DNA was extracted and subjected to bisulfite modification. Methylation status of the gene promoter was studied using quantitative methylation-specific PCR method. Finally, its potential as a diagnostic biomarker was evaluated by receiver operating characteristic curve analysis.
Results: There was not any significant correlation for clinicopathologic features including tumor stage, grade, size, and location with methylation status of OGDHL promoter. However, a significant high methy-lation level was observed in tumoral tissues compared with nontumoral marginal samples (P < 0.0001). Moreover, receiver operating characteristic curve analysis revealed 97.5% sensitivity and 95%, specificity for OGDHL promoter methylation in a cut off of 27.37% methylation as a biomarker for colorectal cancer.
∗ Correspondence to: Dept. of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.
E-mail address: [email protected] (M. Khalaj-kondori).
Please cite this article as: M. Khalaj-kondori, M. Hosseinnejad and A. Hosseinzadeh et al., Aberrant hypermethyla-tion of OGDHL gene promoter in sporadic colorectal cancer, Current Problems in Cancer, https://doi.org/10.1016/j. currproblcancer.2019.03.001
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